Table 2 Primer sequences for quantitative real-time PCR
Target | Nucleotide sequence (5′–3′) | Efficiency | Tm (℃) | Amplicon size (bp) | Correlation coefficients (r2) |
|---|---|---|---|---|---|
GATA3 | AAACCCGATGGATCTGTG TGTGAGTCTGAATGGCTTATT | 110% | 51.26 | 141 | 0.9901 |
IRF4 | TCGAGAAGGCATCGACAAGC GCTCTTGTTCAGAGCACACC | 114% | 56.14 | 70 | 0.9996 |
NFAT | CCCAGGAGAAACAGCATAAA TCTGATCCAGGGCGAGAC | 118% | 54.51 | 105 | 1 |
PU.1 | GCAAGAAGAAGATCCGCCTGTACC TTGTGCTTGGACGAGAACTGGAAC | 90% | 60.05 | 121 | 0.9903 |
SMAD | TTTGTGGGTCGGATCATGGG CAGGGTCCACCGATTCCAAA | 120% | 56.01 | 150 | 1 |
STAT1 | ATGCCACCGAACTTACCCAG AGCTGATCCAGGCAAGCATT | 110% | 56.52 | 112 | 0.9997 |
STAT6 | AGAGGGAGACGACAACAGAG CAGTCACCCAGGAGATGC | 91% | 56.08 | 150 | 0.9995 |
STAT5 | GGCTTTCTTTCTCATTTCC CAACACTCCACCCACCCT | 101% | 52.50 | 107 | 0.9901 |
NF-κB | CCACGTCACATCCAACCC GCTGGCATCAAGCGAAAA | 102% | 55.29 | 159 | 0.9979 |