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Figure 1 | Veterinary Research

Figure 1

From: Binding of Trichinella spiralis C-type lectin with syndecan-1 on intestinal epithelial cells mediates larval invasion of intestinal epithelium

Figure 1

Identification of rTsCTL. A SDS-PAGE analysis of rTsCTL. Lane M: protein marker; Lane 1: lysate of bacteria carrying pGEX-4T-1/TsCTL prior to induction; Lane 2: lysate of bacteria carrying pGEX-4T-1/TsCTL after induction; Lane 3: purified rTsCTL (black arrow). B Western blotting analysis of rTsCTL. The lysates of bacteria carrying pGEX-4T-1/TsCTL prior to induction (lane 1) was not recognized by infection serum; lysate of induced bacteria carrying pGEX-4T-1/TsCTL (lane 2), purified rTsCTL (lane 3–6) were recognized with infection serum (lane 2, 3), anti-rTsCTL serum (lane 4) and anti-GST-tag serum (lane 5), but not by normal serum (lane 6).

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