Figure 10

qPCR analysis of transcription levels of syndecan-1 and TJs in Caco-2 cells treated with Stattic. Caco-2 cells were pre-treated with Stattic (10 µM) and then incubated with rTsCTL (5 µg/mL), 0.1% DMSO (Stattic solvent) was used as a negative control. The transcription levels of syndecan-1 (A), occludin (B), claudin-1 (C), and claudin-2 (D) were assessed by qPCR. The transcription levels were calculated with the 2^−ΔΔCt method. β-Actin was used as an internal control. *P < 0.05 compared to the DMSO group. ^#P < 0.05 compared between two groups.