Figure 11

Western blotting analysis of expression levels of syndecan-1, p-STAT3 and TJ in Caco-2 cells after Stattic treatment. A Caco-2 cells were pre-treated with Stattic (10 µM) and then incubated with rTsCTL (5 µg/mL), and 0.1% DMSO (Stattic solvent) was used as a negative control. The expression levels of syndecan-1, p-STAT3, STAT3, occludin, claudin-1, and claudin-2 were ascertained by Western blotting, and β-Actin was used as an internal reference control. B–F Densitometric analysis of the bands obtained in (A) for syndecan-1 (B), p-STAT3/STAT3 (C), occludin (D), claudin-1 (E) and claudin-2 (F) relative to the β-Actin band. *P < 0.01 relative to the DMSO group. ^#P < 0.01 compared between two groups.