Figure 8

qPCR analysis of transcription levels of syndecan-1 and TJs in Caco-2 cells after β-xyloside treatment. Caco-2 cells were pretreated with β-xyloside (5 mM) and then incubated with rTsCTL (5 µg/mL), and IIL SAg and GST tag protein were respectively used as a positive or negative control. The transcription levels of syndecan-1 (A), occludin (B), claudin-1 (C), and claudin-2 (D) were ascertained by qPCR. The transcription levels were calculated with the 2^−ΔΔCt method. β-Actin was used as an internal control. *P < 0.01 compared to the PBS group. ^#P < 0.001 compared between two groups.