Figure 9

Western blot analysis of expression levels of syndecan-1, p-STAT3 and TJs in Caco-2 cells after β-xyloside treatment. A Caco-2 cells were pretreated with β-xyloside (5 mM) and then incubated with rTsCTL (5 µg/mL), and IIL SAg and GST tag protein were respectively used as a positive or negative control. The expression levels of syndecan-1, p-STAT3, STAT3, occludin, claudin-1, and claudin-2 were analyzed by Western blot, and β-Actin was used as an internal reference control. B–F Densitometric analysis of the bands obtained in (A) panel for syndecan-1 (B), p-STAT3/STAT3 (C), occludin (D), claudin-1 (E) and claudin-2 (F) relative to the β-Actin band *P < 0.01 compared with PBS. ^#P < 0.01 compared between two groups.