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Table 1 Specific primer sequences of Caco-2 cells for qPCR

From: Binding of Trichinella spiralis C-type lectin with syndecan-1 on intestinal epithelial cells mediates larval invasion of intestinal epithelium

Genes

Primers

Sequences (5′ end to 3′ end)

Syndecan-1

Forward primer

TCCTGGACAGGAAAGAGGTGCT

 

Reverse primer

TGTTTCGGCTCCTCCAAGGAGT

Occludin

Forward primer

ATGGCAAAGTGAATGACAAGCGG

 

Reverse primer

CTGTAACGAGGCTGCCTGAAGT

Claudin-1

Forward primer

GTCTTTGACTCCTTGCTGAATCTG

 

Reverse primer

CACCTCATCGTCTTCCAAGCAC

Claudin-2

Forward primer

GTGACAGCAGTTGGCTTCTCCA

 

Reverse primer

GGAGATTGCACTGGATGTCACC

β-Actin

Forward primer

CACCATTGGCAATGAGCGGTTC

 

Reverse primer

AGGTCTTTGCGGATGTCCACGT

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Veterinary Research

ISSN: 1297-9716