Figure 2

Representative western blots showing sPMCA of Norwegian CWD and experimental classical scrapie isolates in the moose brain substrate K₁₀₉/Q₁₀₉ variant. Cervid CWD and experimental classical scrapie isolates were subjected to sPMCA using moose K₁₀₉/Q₁₀₉ brain as substrate. After each PMCA round, the amplified products were digested with proteinase K and analyzed by western blotting. After four rounds of PMCA, all the isolates were amplified and characteristic prion bands could be seen in the western blots. Mo moose, C. scrapie experimental classical scrapie, * Unspecific band, kDa molecular weight in kilodalton.