Veterinary Research

Table 3 sPMCA conversion results.

From: Inter- and intra-species conversion efficacies of Norwegian prion isolates estimated by serial protein misfolding cyclic amplification

Brain substrate		Prion isolate
Species	PRNP-genotype	sPMCA amplification
		Reindeer CWD	Moose CWD		Red deer CWD	Sheep scrapie
		wt	wt	Q₁₀₉/Q₁₀₉	E₂₂₆/E₂₂₆	V₁₃₆/V₁₃₆
Reindeer	S₂₂₅/S₂₂₅ (wt)	+ + + +	−	−	+	+ + +
	S₂₂₅/Y₂₂₅	−	−	−	−	−
	Y₂₂₅/Y₂₂₅	−	−	NA	NA	NA
	D₁₇₆/D₁₇₆	−	−	NA	NA	NA
	M₂S₁₂₉M₁₆₉/M₂S₁₂₉M₁₆₉	−	−	NA	NA	NA
Moose	K₁₀₉/K₁₀₉ (wt)	+ + +	+ +	-	+ + +	+ + +
	Q₁₀₉/Q₁₀₉	+ + + +	+ + +	+ +	+ + +	+ + + +
	K₁₀₉/Q₁₀₉	+ + + +	+ + +	+ + +	+ + +	+ + +
Red deer	Q₂₂₆/Q₂₂₆ (wt)	+ + +	+ +	−	+ +	+ + + +
Red deer	E₂₂₆/E₂₂₆	+ + + +	−	−	+ +	+ + + +
Roe deer	Q₂₂₆/Q₂₂₆ (wt)	+ +	−	−	+ +	+ + +
Sheep	A₁₃₆R₁₅₄Q₁₇₁/A₁₃₆R₁₅₄Q₁₇₁ (wt)	+	−	−	−	+ + + +
	V₁₃₆R₁₅₄Q₁₇₁/V₁₃₆R₁₅₄Q₁₇₁	+	−	−	−	+ + +
	A₁₃₆R₁₅₄R₁₇₁/A₁₃₆R₁₅₄R₁₇₁	−	−	−	−	−
Goat	Q₂₂₀/L₂₂₀	−	−	−	−	+
Goat	Ter₃₂/Ter₃₂	−	−	−	−	−

The grading indicates the PMCA round of first detectable amplification by western blot, following digestion with proteinase K (100 µg/mL final concentration): + , round 7–8; + + , rounds 5–6; + + + , round 3–4; + + + + , round 1–2;—– no amplification; NA not performed, wt wild type, V₁₃₆/V₁₃₆—V₁₃₆ R₁₅₄ Q₁₇₁/V₁₃₆ R₁₅₄ Q₁₇₁, Ter_32/Ter₃₂ no expression of PrP^C.

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