Table 2 Experimental design.
Experiment | Inocula | Duration (dpi) | Birds per group | Follow-up |
|---|---|---|---|---|
1 Pathogenicity assessment | Vv1, Vv2, Cla | 21 | 25 | - Daily clinical score up to 10 dpi |
Im1, Im2, Mock | 20 | |||
2 Evaluation of new parameters | Vv1, Vv2, Cla | 4 | 25 (10 animals were euthanized at 2 dpi and the remaining animals were used at 4 dpi: 10 for Cla, 11 for Vv1 and Vv2 groups) | - Blood samples on tubes with EDTA: Total blood cell count at 2 and 4 dpi - Bursa samples: Bursa to body weight ratios at 2 and 4 dpi and histological analysis -Spleen samples: Spleen to body weight ratios at 2 and 4 dpi - Blood samples on serum tubes: Uric acid dosage - Daily clinical score up to 4 dpi |
Im1, Im2, Mock 2 | 20 (10 of them were euthanized at 2 dpi and the remaining animals were used at 4 dpi: 10 for Im1, Im2 and Mock groups) | |||
3 Evaluation of new parameters | i vaccine, i + vaccine, Mock 1 | 4 | 20 (10 of them were euthanized at 2 dpi and the remaining animals were used at 4 dpi: 10 for i vaccine, i + vaccine and Mock groups) | - Blood samples on tubes with EDTA: Total blood cell count at 2 and 4 dpi - Bursa samples: Bursa to body weight ratios at 2 and 4 dpi and histological analysis -Spleen samples: Spleen to body weight ratios at 2 and 4 dpi - Blood samples on serum tubes: Uric acid dosage - Daily clinical score up to 4 dpi |