Figure 2

A-LS cells affect epithelial barrier function and multiciliated cells. A Schematic representation of the airway epithelial monolayers treatment and processing protocol with alive (A-LS) and inactivated (i-LS) bacteria. B, C Scanning electron microscopy images of airway epithelial cells treated with A-LS (B) or i-LS (C). D Transepithelial resistance (TEER) measurements were used to test the epithelial barrier function in airway control epithelial cells or treated with A-LS or i-LS. E–H Images of live cells in control conditions (E and G), treated with A-LS (F) or treated with i-LS (H). I, J Maximal projection of confocal images for acetylated tubulin (in green), phalloidin (in red) and nucleus (in blue) in controls (I) and A-LS treated cells (J). K Maximal projection of confocal images for acetylated tubulin (in red), phalloidin (in green) and nucleus (in blue) in i-LS treated cells. Scale bar in B and C represents 5 μm. Scale bar in E, F, G and H represents 50 μm. Scale bar in I, J, and K represents 20 μm.