Figure 4

Inactivated BAL 5 treatment reverts the transcriptional program induced by Pasteurella multocida infection in the airway epithelia. A Schematic representation of the airway epithelial monolayers infection, treatment and processing protocol. B Maximal projection of confocal images for acetylated tubulin (in red), phalloidin (in green) and nucleus (in blue) in airway epithelia infected with P. multocida and treated with i-LS. C Number of differentially expressed transcripts in different experimental conditions and comparisons. (D) tSNE result of the analyses of the 15 RNA-seq samples from different conditions. E Table including transcripts related with immune system in different experimental conditions. Red box indicates upregulated transcripts in cells treated with i-LS and infected with P. multocida, which were downregulated in P. multocida infected cells. Blue box indicates downregulated transcripts in P. multocida infected cells, which were not differentially expressed in cells treated with i-LS treated and infected with P. multocida. Scale bar in B represents 20 μm.