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Figure 1 | Veterinary Research

Figure 1

From: Development and application of classical swine fever virus monoclonal antibodies derived from single B cells

Figure 1

SDS-PAGE and Western blot analysis of CSFV E2. A Recombinant E2 proteins were expressed using SF9 insect cells, and the molecular weight of the expressed E2 protein was 43 kDa. Lane 1, BSA (2 µg); Lane 2, purified E2 (reducing conditions, 2 µg). B Purified E2 protein was analysed by Western blotting using a mouse anti-His mAb (GenScript), and dimer bands were observed. C Highly purified E2 protein was biotinylated with EZ-Link Sulfo-NHS-LC-biotin reagent, and biotinylated E2 was recognized by streptavidin-HRP (GenScript). Lane 1, 0.1 µg; Lane 2, 0.2 µg. D Purified E2 protein was further analysed by Western blotting using CSFV-positive serum, and the expressed E2 protein was accurately recognized by specific antibodies.

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Veterinary Research

ISSN: 1297-9716