Skip to main content

Table 1 Primers used for PCR amplification of the gamma chain of murine IgG

From: Development and application of classical swine fever virus monoclonal antibodies derived from single B cells

Primers

Primer sequencea (5′ → 3′)

Igγ forward

AGAGTGCTGATTCTTTTGTG

GAATGGCCTTATATCTTTCTC

GAATGGAGTTGGATATTTCTC

TCCTTGAATCTTTCTCTTCCT

GGATTCAGCAGGATCTTTCTC

GGGACACAGGACCTCACCAT

GGATGGAGCTGTATCATCCTC

GGATGGAGCTGTTTCATCGTC

GGATTGCTGTTTCTTTTCTTC

GAATGGCTGTGGAACTTGCT

Igγ reverse outer

GAAGGTGYGCACACYGCTGGAC (1st PCR)

Igγ reverse inner

GCTCAGGGAARTAGCCCTTGAC (2nd PCR)

  1. aDegenerate bases, including S = C or G, Y = C or T, and R = A or G, were synthesized in these sequences.
Back to article page

Veterinary Research

ISSN: 1297-9716