Development and application of classical swine fever virus monoclonal antibodies derived from single B cells

Table 2 Primers used for PCR amplification of the lambda chain of murine IgG

Primers	Primer sequence^a (5′ → 3′)
Igλ forward	GAGACAGACACAATCCTGCTA GATTTTCAAGTGCAGATTTTC AAGTTGCCTGTTAGGCTGTTG GATTCACAGGCCCAGGTTCTT GAATCACAGACTCAGGTCCTC TTACTGCTGCTATGGGTATCT ACCATGTTCTCACTAGCTCTT TTCTGGATTCCTGCTTCCAGC AGGGTCCTTGCTGAGCTCCTG GAGACAGACACACTCCTGTTA GAGACAGACACACTCCTGCTA ACCATGCTCTCACTAGCTCCT
Igλ reverse outer	GTACCATYTGCCTTCCAGKCCACT (1st PCR)
Igλ reverse inner	CTCYTCAGRGGAAGGTGGRAACA (2nd PCR)

^aDegenerate bases, including S = C or G, Y = C or T, and R = A or G, were synthesized in these sequences.

ISSN: 1297-9716