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Table 3 Primers used for PCR amplification of the kappa chain of murine IgG

From: Development and application of classical swine fever virus monoclonal antibodies derived from single B cells

Primers

Primer sequencea (5′ → 3′)

Igκ forward

GCTGTTTTGTATACCTGGG

CTCAGCTCCTGTTGCTGTGGC GTGTMTGGTGCTBRTGGG

GGGTATCTGGTACCTGTGG

CCTCATATTTTTGCTGCTATGGG

TGCTTTTCTGGATTTCAGCCTCC

TCAACTTCTGCTCTTCCTGCTGT

CTAGCTCYTCTCCTCAGYCTTCTT

Igκ reverse outer

ACTGAGGCACCTCCAGATG (1st PCR)

Igκ reverse inner

TGGGAAGATGGATACAGTT (2nd PCR)

  1. aDegenerate bases, including S = C or G, Y = C or T, and R = A or G, were synthesized in these sequences.
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Veterinary Research

ISSN: 1297-9716