Figure 1

Schematic representation of the model viruses and LC‒MS/MS spectra obtained in this study. A Three NDV strains were utilized as model viruses in this study: the vaccine strain Mukteswar, the vaccine variant strain JS/7/05/Ch, and the HN replacement strain JS/MukHN, which is a derivative of JS/7/05/Ch carrying the HN gene from Mukteswar. The aa mutations in NDV NP and HN are marked with yellow triangles and include P438S in NP and N19S, S29T, M145T, V266I, A494D, and E495K in HN. B The LC‒MS/MS spectra were obtained for the immunoprecipitated protein samples. The protein samples obtained from the immunoprecipitates were prepared after the infection of JS/7/05/Ch and JS/MukHN. Subsequently, the samples underwent further processing and analysis using the LC‒MS/MS system. Coimmunoprecipitation assays were performed to investigate the interaction between NDV HN and vimentin.