Figure 3

The regulation of vimentin rearrangement by NDV infection. NDV infection induces differential vimentin rearrangement in HD11 cells (A) and CEFs (B). Cells were infected with JS/7/05/Ch, JS/MukHN, or Mukteswar at an MOI of 1 for 6, 12, and 18 h. The infected cells were fixed with cold 4% paraformaldehyde, permeabilized with Triton X-100, and blocked with 5% BSA. Vimentin (red) was subsequently visualized using an anti-vimentin antibody on a confocal microscope, with the nuclei stained with DAPI (blue). The mock-infected cells were assayed in parallel as a control. Images were captured at different magnifications to obtain more precise fields of view. The white arrowheads indicate rearranged vimentin. Scale bars, 10 μm.