Figure 4

Reciprocal dependency between NDV infection and vimentin rearrangement. NDV infection induces differential vimentin rearrangement in HD11 cells (A) and CEFs (B). Cells were infected with non-UV-inactivated NDV or UV-inactivated NDV at an MOI of 1 for 18 h and were also pretreated with IDPN prior to being infected with NDV. The infected cells were then fixed with cold 4% paraformaldehyde, permeabilized with Triton X-100, and blocked with 5% BSA. NDV dsRNA (green) and vimentin (red) were visualized using specific antibodies on a confocal microscope, with the nuclei stained with DAPI (blue). Images were captured at 630 × magnification. The white arrowheads indicate rearranged vimentin. The white dashed box indicates colocalization of rearranged vimentin filaments and viral dsRNA. Scale bars, 10 μm.