Figure 7

Effects of vimentin on the attachment of NDV. HD11 cells in 6-well cell culture plates were pretreated with an anti-vimentin antibody or normal rabbit IgG and then infected with NDV (MOI = 1) or treated with PBS at 4 ℃ for 1 h. The infected cells were washed with ice-cold PBS solution (pH = 7.2) containing 2% FBS. Then, the cells were incubated with a mouse anti-NDV HN primary antibody at 4 ℃ overnight and a FITC-conjugated secondary antibody at 37 ℃ for 1 h. The mock-infected cells were assayed in parallel as a control. The cells with NDV attachment were finally detected by flow cytometry, and flow cytometry scatter plots were generated using CytExpert 2.3 software. Positive cell populations were established based on granularity (SSC-A) and the FITC-A fluorescence channel. The ratio of the number of positive cells to the total number of cells was calculated and is displayed within the box. The statistical graphs were generated using GraphPad Prism 9.0 software. ns indicates no significance.