Figure 2

Salmonella Typhimurium gene expression under normoxia and hypoxia conditions. A. Salmonella’s fimH gene expression. Relative expression of fimH mRNA in STmWT and its mutant in normoxia (dark gray bars) and hypoxia (light gray bars) after the third passage of static growth in Luria–Bertani (LB) broth. Data represent the mean ± SD of three independent experiments. Triplicate samples were analyzed in each experiment to confirm the accuracy and reproducibility of qPCR. Statistical differences were analyzed by Student t-test (A) and are presented as the mean. ****p < 0.0001. B–E Vulcano plots for differentially expressed genes (DEG) in STmWT STmΔfimH. The green spots represent the downregulated genes, whereas the red spots represent upregulated genes. The blue spots indicate genes without significantly different expression levels between the two groups. P values for each point are indicated on the y-axis, lod2 of expression fold change indicated on the x-axis. B Comparison between STmWT grown in normoxia versus hypoxia; C Comparison between STmΔfimH grown in normoxia versus hypoxia. D Comparison between STmWT and STmΔfimH grown in normoxia; E Comparison between STmWT and STmΔfimH grown in hypoxia.