Figure 2
From: 3D sheep rumen epithelial structures driven from single cells in vitro
Rumen organoids cultured in minus medium. A Diameter variation of organoids. B Organoid formation rates of rumen epithelial cells after multiple passages. C, D Formation rates and diameters of organoids cultured in minus medium (different superscript letters indicate significant differences, P < 0.05). E, F Organoids cultured in minus medium. G Inhibitor removal from OCM for 3 days. H Representative images showing organoids following ten passages. Scale bars = 100 μm. Data and images are representative of three independent experiments using three individual lambs.