Figure 4
From: 3D sheep rumen epithelial structures driven from single cells in vitro
Ultrastructural analysis and expression of mRNA encoding cell junction-related genes in rumen epithelial tissue and organoids. A Transmission electron microscopy (TEM) showing the tight junctions, desmosomes, and gap junctions between cells in the outer layer of the organoid and the mitochondria in the cytoplasm. B TEM showing the tight junctions between cells and the mitochondria inside the organoid, with gaps of a few micrometers between cells. C TEM showing the dense corneodesmosomes between cells in the inner layer of the organoid. D TEM showing the tight junctions and gap junctions between cells inside organoids. E Heatmap showing the relative expression levels (log2 transcripts/million reads) of a range of epithelial cell junction-related genes. GJ, gap junction. M, Matrigel; Black arrow, desmosome; White arrow, gap junction; Black star, mitochondria; White star, corneodesmosomes; White triangle, tight junction. Representative TEM images of one experiment. The expression data include rumen tissue and rumen organoids from 3 lambs at 5 days of age.