Table 2 List of reagents to prepare BM and OCM
From: 3D sheep rumen epithelial structures driven from single cells in vitro
BM | Final | OCM (BM +) | Final |
|---|---|---|---|
Advanced DMEM/F-12 | Â | EGF | 50 ng/mL |
Penicillin/streptomycin | 1 × | Noggin | 100 ng/mL |
N2 | 1 × | Wnt3a | 100 ng/mL |
B27 | 1 × | R-spondin1 | 100 ng/mL |
GlutaMax | 1 × | IGF-1 | 100 ng/mL |
HEPES | 1 × | FGF-10 | 100 ng/mL |
Nicotinamide | 10 mM | CHIR-99021 | 3 µM |
N-Acetyl-L-Cysteine | 1 mM | A83-01 | 5 µM |
|  |  | SB202190 | 10 µM |
|  |  | Y-27632 | 10 µM |