Skip to main content
Figure 1 | Veterinary Research

Figure 1

From: Duck Tembusu virus induces incomplete autophagy via the ERK/mTOR and AMPK/mTOR signalling pathways to promote viral replication in neuronal cells

Figure 1

DTMUV infection in Neuro-2a cells. Neuro-2a cells were infected with DTMUV for 24 h, 36 h and 48 h. A Detection of DTMUV infection in Neuro-2a cells by indirect immunofluorescence assays. Green fluorescence indicates that cells were infected with DTMUV strain AQ-19. Scale bar, 20 μm. B Western blotting analysis of DTMUV infection. E protein levels were detected, and band intensities were analysed using ImageJ. C qRT-PCR detection of DTMUV proliferation. Total RNA of cells was extracted for measurement of viral copies through qRT-PCR assay. D The viability of Neuro-2a cells was evaluated by CCK-8 assays. The results are shown as the means ± SDs of three independent experiments. Significant differences were determined with one-way ANOVA. ns: not significant; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Back to article page

Veterinary Research

ISSN: 1297-9716