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Figure 5 | Veterinary Research

Figure 5

From: Duck Tembusu virus induces incomplete autophagy via the ERK/mTOR and AMPK/mTOR signalling pathways to promote viral replication in neuronal cells

Figure 5

DTMUV infection induces incomplete autophagy in the mouse brain, which contributes to viral replication and neuropathogenesis. A Autophagosome-like vesicles (blue arrows) were observed in the mouse brains by TEM. Scale bar, 1 μm. B Protein levels of LC3-II and P62 in the brain tissues of mice infected with DTMUV for 2 dpi, 4 dpi and 6 dpi were determined by Western blotting, and band intensities were analysed. C qRT-PCR analysis of DTMUV copies in the brains of mice from the AQ-19, RAPA+AQ-19 and 3-MA+AQ-19 groups. D Immunohistochemical (IHC) staining was used to observe the distribution of DTMUV antigen (brown) in the brains of mice from the control, AQ-19, RAPA+AQ-19 and 3-MA+AQ-19 groups. Scale bar, 100 μm. E Histopathological examination of the brain tissues of mice from the control, AQ-19, RAPA+AQ-19 and 3-MA+AQ-19 groups. Scale bar, 200 μm. The results are presented as the means ± SDs of three experiments. Differences were analysed using one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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Veterinary Research

ISSN: 1297-9716