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Figure 1 | Veterinary Research

Figure 1

From: The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity

Figure 1

Generation and Characterization of ASFV CD2v-expressing stable macrophage cell line. A Morphology of PAM-CD2v and PAM-GFP cell lines. Scale bars: 200 μm. B Representative images for immunofluorescence staining of Flag tag for CD2v in PAM-CD2v and PAM-GFP cell lines. Scale bars: 200 μm. C CD2v protein levels were determined by Western blotting. D PAM-CD2v macrophages hemadsored swine red blood cells (RBCs). PAM-CD2v and PAM-GFP macrophages were subjected to overnight incubation with swine RBCs, followed by examination through light microscopy. The arrowheads indicate CD2v-dependent rosetting. Scale bars: 1000 μm.

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Veterinary Research

ISSN: 1297-9716