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Figure 3 | Veterinary Research

Figure 3

From: The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity

Figure 3

CD2v protein inhibits swine macrophage migration. A Gene set enrichment analysis (GSEA) plot of locomotory behavior gene set. Rank ordered by log2Foldchange of PAM-CD2v versus PAM-GFP. B Representative images of PAM-GFP and PAM-CD2v migration at 24 h and 48 h. Scale bar: 200 μm. C The number of migratory cells was evaluated by five random fields’ relative staining areas (***, P < 0.001, n = 5). D After an initial scratch, representative images of PAM-GFP and PAM-CD2v cells at 12 and 24 h. E Quantification of scratch width in PAM-GFP and PAM-CD2v macrophages (***, P < 0.001; ns, not significant; n = 6 per group). (F) Cell proliferation of PAM-GFP and PAM-CD2v macrophages was determined by resazurin at different time points as indicated (ns, not significant; n = 8).

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Veterinary Research

ISSN: 1297-9716