Skip to main content
Figure 4 | Veterinary Research

Figure 4

From: The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity

Figure 4

CD2v downregulates EGR1 by inhibiting the activity of ERK1/2. A Heatmap of all annotated swine genes in the locomotory behavior gene set. EGR1 is highlighted in red. B EGR1 expression in PAM-GFP and PAM-CD2v macrophages for RNA-seq (***, P < 0.001; n = 3). C EGR1 expression in PAM-GFP and PAM-CD2v macrophages was evaluated by RT-qPCR with specific primers (***, P < 0.001; n = 3). D EGR1 protein levels were determined by Western blotting in PAM-GFP and PAM-CD2v macrophages. E GSEA plots of extracellular matrix organization and extracellular matrix assembly gene sets. Rank ordered by log2Foldchange of PAM-CD2v versus PAM-GFP. F Representative Western blotting bands for CD2v, EGR1, ERK1/2, phosphorylation ERK1/2, and Tublin in PAM-GFP and PAM-GFP; G PAMs were treated with ERK1/2 inhibitor FR180204 or vehicle DMSO. The expression of EGR1 and phosphorylation ERK1/2 were evaluated by Western blotting.

Back to article page

Veterinary Research

ISSN: 1297-9716