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Figure 5 | Veterinary Research

Figure 5

From: The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity

Figure 5

EGR1 depleted in macrophage reduces cell migration. A Schematic illustration of EGR gene locus of swine and small guide RNA location. The EGR1 expression was determined by Western blotting in EGR1-WT PAMs and EGR1-KO1 PAMs. B Representative images of EGR1-WT and EGR1-KO1 PAMs migration at 24 h and 48 h. Scale bar: 200 μm. C The number of migratory cells from the transwell assay was evaluated by five random fields’ relative staining areas (*** P < 0.001; n = 5). D Representative images of EGR1-WT and EGR1-KO1 PAMs at 12 and 24 h after initial scratch. E Quantification of scratch width EGR1-WT and EGR1-KO1 PAMs at different time points as indicated (** P < 0.01; *** P < 0.001; ns, not significant; n = 6). F Cell proliferation of EGR1-WT and EGR1-KO1 PAMs was determined by resazurin at different time points as indicated (ns, not significant; n = 8).

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Veterinary Research

ISSN: 1297-9716