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Figure 6 | Veterinary Research

Figure 6

From: The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity

Figure 6

EGR1 overexpression in PAM-CD2v restores the ability of cell migration. A The EGR1 protein was determined by Western blotting in PAM-CD2v-EGR1 and PAM-CD2v-GFP cells. B Representative images of cell migration from PAM-CD2v-EGR1 and PAM-CD2v-GFP cells at 24 h and 48 h. C The number of migratory cells from the transwell assay was evaluated by five random fields’ relative staining areas (*** P < 0.001; n = 5). D Representative images of PAM-CD2v-EGR1 and PAM-CD2v-GFP cells at 12 and 24 h after initial scratch. E Quantification of scratch width of PAM-CD2v-EGR1 and PAM-CD2v-GFP cells at different time points as indicated (** P < 0.01; *** P < 0.001; ns, not significant; n = 6). F Cell proliferation of PAM-CD2v-EGR1 and PAM-CD2v-GFP were determined by resazurin at different time points as indicated (ns, not significant; n = 8).

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Veterinary Research

ISSN: 1297-9716