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Figure 7 | Veterinary Research

Figure 7

From: The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity

Figure 7

ChIP-Seq analysis of genome-wide binding sites of EGR1 in PAMs. A Distribution of EGR1-binding and H3K27ac enrichment sites in PAMs. Chromatin immunoprecipitation was conducted followed by deep sequencing (ChIP-seq) with the use of an EGR1 antibody and H3K27ac antibody. The total number of EGR1-bound and H3K27ac enrichment peaks was identified, and the percentage found within various genome regions was calculated. B Profile plots and heatmaps of the distribution of EGR1-binding and H3K27ac from ± 3 kb to genes’ transcriptional start site (TSS) are shown. The x-axis denotes the position from the TSS, and the y-axis shows the signal strength of 30 440 swine genes. C Overlayed profile plot of EGR1 and H3K27ac ChIP-seq read distribution within 3 kb of TSS. D Quantitative correlation of signal strength of EGR1 binding and H3K27ac enrichment at all genes TSSs. The linear regression trendline and the correlation coefficient-square are displayed.

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Veterinary Research

ISSN: 1297-9716