Figure 3

Chicken mimic miR-148a represses several target genes in the MAPK signalling pathway and antiviral response. A Expression profiles of gga-miR-148a-3p and B its candidate target genes in the HD11 chicken macrophage line transfected with mimic miR-148a-3p or NC-miR treated with and Poly I:C. C Western blot analysis of p-p38 and GAPDH proteins in the HD11 chicken macrophage line transfected with mimic miR-148a. The levels of miR-148a were analysed by RT-qPCR and normalized to those of endogenous U1A RNA. Gene expression levels were normalized to those of GAPDH. Data are presented as the mean ± SEM (n = 3) from three independent experiments. Six treatment groups were included in this experiment: (1) no miRNA, no Poly I:C, (2) mimic gga-miR-NC transfection, (3) mimic gga-miR-148a-3p transfection, (4) Poly I:C treatment, (5) mimic gga-miR-NC transfection and Poly I:C treatment, and (6) mimic gga-miR-148a-3p transfection and Poly I:C treatment. Different lowercase letters above the dots indicate significant differences (p < 0.05) among samples as determined by analysis of variance with Tukey’s multiple comparison test.