Figure 4

Luciferase reporter assay. A Schematic diagram of miRNA binding sites and wild-type and mutant target genes (IFN-γ/Luc, Mut-IFN-γ/Luc, MAPK11/Luc, Mut-MAPK11/Luc, TGF-β2/Luc, and Mut-TGF-β2/Luc). The 3′-UTRs of wild-type and mutant target genes were cloned and inserted into a pMIR-REPORT luciferase vector. B Luciferase reporter assay performed at 24 h following cotransfection of chicken embryo fibroblast line (DF1) with the wild type or mutant vectors together with mimic miR-148a-3p or NC-miR as control. The results (mean ± SEM) are representative of three independent experiments (n = 3). Different lowercase letters above the dots indicate significant differences (p < 0.05) among samples as determined by analysis of variance with Tukey’s multiple comparison test.