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Figure 4 | Veterinary Research

Figure 4

From: ProQ binding to small RNA RyfA promotes virulence and biofilm formation in avian pathogenic Escherichia coli

Figure 4

Role of ProQ in APEC/ExPEC replication within macrophages. A The intracellular replication and survival of WT FY26 cells in RAW264.7 macrophages. The data represent the cumulative results from at least four individual experiments. Significant differences were analysed using two-way ANOVA (*P < 0.01). B Intracellular replication and survival of mutant FY26ΔproQ within HD11 macrophages. C Evaluation of the impact of proQ deletion on APEC intracellular proliferation in HD11 cells was determined using an immunofluorescence assay. Quantitative data for bacterial proliferation were derived from approximately 200 infected cells across at least three independent experiments. D Comparative intracellular proliferation of WT FY26, FY26ΔproQ, and FY26CproQ strains in HD11 macrophages assessed at 8 hpi. Infected HD11 cells were fixed with DAPI and labelled with phalloidin. Bacteria within the cells were targeted with anti-FY26 antibody followed by labelling with FITC-conjugated secondary antibody. Scale bar represents 10 μm.

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Veterinary Research

ISSN: 1297-9716