Figure 6

Direct interaction of ProQ with the sRNA RyfA. A The transcription profiles of WT FY26 and the mutant FY26ΔproQ were compared. B Canonical RNA structure associated with ProQ binding at intrinsic terminators, as detailed in [33]. C Predicted secondary structure of RyfA sRNA. D Temporal Northern blotting assessment of RyfA transcription across various growth stages in LB medium at 37 °C of WT FY26, FY26ΔryfA, and FY26CryfA. E Validation of the interaction between ProQ and the sRNA RyfA through EMSA. Notably, no interaction was observed between ProQ and the RyfA variant lacking a poly(U) tail, even as the ProQ concentration increased. The binding of sRNA RbsZ to ProQ served as a positive control.