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Figure 4 | Veterinary Research

Figure 4

From: LppA is a novel plasminogen receptor of Mycoplasma bovis that contributes to adhesion by binding the host extracellular matrix and Annexin A2

Figure 4

Binding of M. bovis LppA to host ECM components. A–D Binding ability of M. bovis LppA to host extracellular matrix components (fibronectin, collagen IV, laminin, and vitronectin) was tested by dot blot, with 6 × His peptides as the negative control. E–H LppA binds to fibronectin, collagen IV, laminin, and vitronectin in a dose-dependent manner. Different concentrations of rLppA (250–1.95 ng) were added to wells coated with fibronectin, collagen IV, laminin, or vitronectin (200 ng). The optical density was measured at 405 nm.

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Veterinary Research

ISSN: 1297-9716