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Figure 7 | Veterinary Research

Figure 7

From: LppA is a novel plasminogen receptor of Mycoplasma bovis that contributes to adhesion by binding the host extracellular matrix and Annexin A2

Figure 7

LppA promotes the enrichment of ANXA2 on the cell membrane. The pCAGGS-FLAG-N and pCAGGS-FLAG-N-LppA plasmids were separately transfected into embryonic bovine lung (EBL) cells for 24 h. A Expression changes of ANXA2 in the cell membrane and cytoplasm were detected using an anti-ANXA2 antibody. α-Tubulin and Na+/K+-ATPase were used as marker proteins for the cytoplasm and cell membrane, respectively. B Relative ANXA2 expression in the cell membrane and cytoplasm. C EBL cells transfected with the pCAGGS-FLAG-N plasmid. D EBL cells transfected with the pCAGGS-FLAG-N-LppA plasmid. Blue represents DAPI, red represents ANXA2, and green represents rLppA. The white arrow indicates the enrichment of the ANXA2 protein on the cell membrane.

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Veterinary Research

ISSN: 1297-9716