Figure 8

LppA is a novel adhesion protein of M. bovis. A Presence of LppA in M. bovis wild-type, M. bovis ΔLppA, and M. bovis ΔLppA:LppA was examined using anti-LppA mouse serum. B Comparative growth curves of M. bovis wild-type, M. bovis ΔLppA, and M. bovis ΔLppA:LppA. C M. bovis wild-type, M. bovis ΔLppA, and M. bovis ΔLppA:LppA were used to infect embryonic bovine lung (EBL) cells for 1.5 h. Subsequently, the cells were washed three times with phosphate-buffered saline, digested with trypsin, and the adherent mycoplasmas were counted. D Adhesion inhibition assay of anti-LppA serum to M. bovis. M. bovis was pre-incubated with various concentrations of anti-LppA serum for 1 h and then used to infect EBL cells. Pre-immune serum was used as the control. Values are presented as mean ± standard error of the mean of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001). E M. bovis wild-type, ΔLppA, and ΔLppA:LppA were stained with CFDA-SE for 30 min and then used to infect EBL cells. The nuclei of the cells were stained with DAPI and the cytoskeleton was stained with rhodamine phalloidin. Green represents M. bovis, red represents the cytoskeleton, and blue (DAPI) represents the nucleus.