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Figure 1 | Veterinary Research

Figure 1

From: Trichinella spiralis galectin binding to toll-like receptor 4 induces intestinal inflammation and mediates larval invasion of gut mucosa

Figure 1

Expression and identification of rTsgal. A SDS-PAGE analysis of rTsgal, lane M: protein marker; Lane 1: Lysate of recombinant E. coli incorporating pGEX-4T-1/Tsgal prior to induction; Lane 2: Lysate of recombinant E. coli incorporating pGEX-4T-1/Tsgal following induction; Lane 3: rTsgal expressed in lysate supernatant of recombinant E. coli incorporating pGEX-4T-1/Tsgal following induction; Lane 4: rTsgal expressed in lysate precipitate of recombinant E. coli incorporating pGEX-4T-1/Tsgal following induction. B SDS-PAGE analysis of IIL antigens and purified rTsgal. Lane M: protein marker; lane 1: IIL crude antigens; lane 2: IIL ES antigens; lane 3: purified rTsgal. C Western blotting analysis of rTsgal antigenicity. Lane M: protein marker. IIL crude antigens (lane 1, 4) and rTsgal (lane 3, 6) were recognized by infection serum (lane 1, 3) and anti-rTsgal serum (lane 4, 6), but IIL ES antigens (lane 2, 5) were not recognized by infection serum (lane 2) and anti-rTsgal serum (lane 5). Lane 7–9: IIL crude, ES antigens and rTsgal were not recognized by normal serum.

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Veterinary Research

ISSN: 1297-9716