Figure 12

TAK-242 and PDTC inhibited the larval invasion of Caco-2 cells. Caco-2 monolayer was first pretreated with 25 µM TAK-242 and 300 mM PDTC at 37 °C for 2 h. The muscle larvae were activated into the IIL using 5% swine bile for 2 h at 37 °C, then added onto the Caco-2 monolayer and larval invasion was examined under microscope at 2 h after co-culture. A The invaded larva was locomotive and migrating in the monolayer. B Non-invaded larva was coiled on the cell surface. C, D TAK-242 (C) and PDTC (D) inhibited the larval invasion. The results are expressed as the mean ± SD of three independent tests. Scale bars: 20 μm. *P < 0.05 compared to the DMSO or PBS group. ^#P < 0.05 compared between the two groups.