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Figure 2 | Veterinary Research

Figure 2

From: Trichinella spiralis galectin binding to toll-like receptor 4 induces intestinal inflammation and mediates larval invasion of gut mucosa

Figure 2

Co-localization of rTsgal and TLR-4 on Caco-2 cell membrane. A Caco-2 cells pre-incubated with rTsgal, IIL CAg and PBS were probed by mouse anti-rTsgal serum, infection serum and normal serum, and Alexa Fluor 488-conjugated anti-mouse IgG as secondary antibody. Caco-2 cells pre-incubated with rTsgal, IIL CAg and PBS were also probed by rabbit anti-TLR-4 antibody, and Cy3-conjugated anti-rabbit IgG was served as the secondary antibody. DAPI: cell nucleus was stained by DAPI as blue. B Enlarged areas of co-localization of rTsgal and TLR-4 on Caco-2 cells. The binding of rTsgal and TLR-4 was co-localized mainly on the cellular membrane as shown as white arrows. Scale bars: 40 μm.

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Veterinary Research

ISSN: 1297-9716