Figure 4

Binding between rTsgal and TLR-4 detected by Co-IP. rTsgal were first incubated with Caco-2 cells. Then, anti-rTsgal antibodies and protein A/G were added and incubated for 12 h. The bound proteins were subjected to SDS-PAGE and transferred to a NC membrane. The membrane was probed with anti-rTsgal antibodies and anti-TLR-4 antibody. Lane 1: pulled down immune complex (rTsgal, anti-rTsgal antibodies and TLR-4 in Caco-2 cell proteins); Lane 2: normal mouse IgG; Lane 3: Caco-2 cell proteins.