Table 1 Specific primer sequences of Caco-2 and mouse makers and cytokines for qPCR
Gene | Primers | Sequence | GenBank no. |
|---|---|---|---|
GAPDH (Human) | Forward Reverse | 5′-GTCTCCTCTGACTTCAACAGCG′-3′ 5′-ACCACCCTGTTGCTGTAGCCAA′-3′ | NM_002046 |
TLR-4 (Human) | Forward Reverse | 5′-AGACCTGTCCCTGAACCCTA′-3′ 5′-CTCCCAGAACCAAACGATG′-3′ | NM_138554 |
NF-κB p65 (Human) | Forward Reverse | 5′-TGAACCGAAACTCTGGCAGCTG′-3′ 5′-CATCAGCTTGCGAAAAGGAGCC′-3′ | NM_021975 |
IL-1β (Human) | Forward Reverse | 5′-CCACAGACCTTCCAGGAGAATG′-3′ 5′-GTGCAGTTCAGTGATCGTACAGG′-3′ | NM_000576 |
IL-6 (Human) | Forward Reverse | 5′-AGACAGCCACTCACCTCTTCAG′-3′ 5′-TTCTGCCAGTGCCTCTTTGCTG′-3′ | NM_000600 |
TGF-β (Human) | Forward Reverse | 5′-TACCTGAACCCGTGTTGCTCTC′-3′ 5′-GTTGCTGAGGTATCGCCAGGAA′-3′ | NM_000660 |
GAPDH (Mouse) | Forward Reverse | 5′-CATCACTGCCACCCAGAAGACTG′-3′ 5′-ATGCCAGTGAGCTTCCCGTTCAG′-3′ | NM_008084 |
TLR-4 (Mouse) | Forward Reverse | 5′-AGCTTCTCCAATTTTTCAGAACTTC′-3′ 5′-TGAGAGGTGGTGTAAGCCATGC′-3′ | NM_021297 |
NF-κB p65 (Mouse) | Forward Reverse | 5′-TCCTGTTCGAGTCTCCATGCAG′-3′ 5′-GGTCTCATAGGTCCTTTTGCGC′-3′ | NM_009045 |
IL-1β (Mouse) | Forward Reverse | 5′-TGGACCTTCCAGGATGAGGACA′-3′ 5′-GTTCATCTCGGAGCCTGTAGTG′-3′ | NM_008361 |
IL-6 (Mouse) | Forward Reverse | 5′-TACCACTTCACAAGTCGGAGGC′-3′ 5′-CTGCAAGTGCATCATCGTTGTTC′-3′ | NM_031168 |
TGF-β (Mouse) | Forward Reverse | 5′-TGATACGCCTGAGTGGCTGTCT′-3′ 5′-CACAAGAGCAGTGAGCGCTGAA′-3′ | NM_011577 |