Figure 1
From: Molecular characteristics and pathogenicity of a novel chicken astrovirus variant
Isolation and identification of CAstV. A LMHs were infected with virus isolates and tested by PCR for various chicken-original viruses. B Cytopathic effects of LMH-infected SDAU2022-1 at 48 hpi. C Growth kinetics of CAstV isolate infected LMHs at 6, 12, 24, 48, and 72 hpi. D Structural representation of CAstV, featuring three open reading frames (ORFs) and characteristic “stem-loop-II-like” motifs. The initiation site of the heptameric AAAAAAC (RFS) sequence is indicated by black triangles. Gray bars are the transmembrane domains. ORF, open reading frame; RFS, ribosomal frameshift signal; s2m, stem-loop-II-motif.