Figure 9

The schematic diagram of PRRSV inhibition by vidofludimus. Dihydroorotate dehydrogenase (DHODH), the key rate-limiting enzyme of the fourth step reaction in the de novo synthesis of pyrimidine (blue arrow), is important in generating UMP required for viral replication. By targeting and inhibiting DHODH activity, vidofludimus (Vi) blocks the production of orotate (ORO) and finally inhibits the synthesis of viral RNA. In addition, the effect of DHODH inhibitors can be disturbed by the salvage pathway (green arrow). On the other hand, Vi can also inhibit PRRSV adsorption on target cells, and its mechanism may be related to PRRSV adsorption-related protein receptors.