Figure 4From: Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickensExpression detection of pVAX1-14EGT in 293 T cells. pVAX1/14EGT + : 293 T cells were transfected with the eukaryotic expression plasmid pVAX1-14EGT and detected by anti-r14EGT rat serum. pVAX1 + : 293 T cells were transfected with the eukaryotic expression vector pVAX1 and detected by anti-r14EGT rat serum. pVAX1/14EGT: 293 T cells were transfected with the eukaryotic expression plasmid pVAX1-14EGT and detected in negative rat serum. pVAX1: 293 T cells were transfected with the eukaryotic expression vector pVAX1 and detected in negative control rat serum. Bright field: view of the laser confocal microscope. DAPI: nuclear staining image. CY3: Cy3-labelled goat anti-rat IgG staining image. Merged: the merged graph of nuclear staining images and Cy3 staining images.Back to article page