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Table 4 Protective efficacies of multiepitope vaccines constructed from common antigens of Eimeria species in chickens.

From: Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens

Trials

Challenged Eimeria species

Groups

Weight gain (g)

Mean lesion score

Mean OPG × 105

Oocyst decrease rate %

ACI

Trial 1

E. maxima

Unchallenged control*

44.14 ± 8.04a

0 ± 0a

0 ± 0a

100

200

Challenged control

19.97 ± 5.70b

2.27 ± 0.68bc

1.49 ± 0.044b

0

83.02

pVAX1 control

23.15 ± 8.35b

2.33 ± 0.60bc

1.40 ± 0.26b

6.26

88.63

pET-32a tag protein control

23.69 ± 7.61b

2.53 ± 0.50b

1.10 ± 0.49c

26.49

102.4

pVAX1-14EGT

40.99 ± 11.54a

1.93 ± 0.68 cd

0.32 ± 0.11d

78.83

173.17

r14EGT

39.78 ± 8.20a

1.73 ± 0.57d

0.37 ± 0.08d

74.76

162.4

Trial 2

E. acervulina

Unchallenged control*

44.14 ± 8.04a

0 ± 0a

0 ± 0a

100

200

Challenged control

27.26 ± 9.02b

2 ± 0.73b

0.28 ± 0.07b

0

102.69

pVAX1 control

26.19 ± 7.33b

1.6 ± 0.49c

0.21 ± 0.05c

25.24

124.35

pET-32a tag protein control

26.87 ± 9.34b

1.6 ± 0.49c

0.19 ± 0.04c

34.21

124.96

pVAX1-14EGT

40.73 ± 7.70a

1.4 ± 0.49c

0.08 ± 0.01d

62.69

169.87

r14EGT

40.20 ± 11.08a

1.27 ± 0.44c

0.11 ± 0.02e

62.17

168.11

Trial 3

E. tenella

Unchallenged control#

41.05 ± 16.08a

0 ± 0a

0 ± 0a

100

200

Challenged control

8.59 ± 6.37b

2.53 ± 0.50b

6.38 ± 1.03b

0

76

pVAX1 control

14.3 ± 5.81b

2.87 ± 0.50b

5.91 ± 0.44bc

7.4

91.41

pET-32a tag protein control

11.15 ± 8.31b

2.8 ± 0.75b

5.41 ± 1.89bc

15.28

84.36

pVAX1-14EGT

37.11 ± 12.18a

1.33 ± 0.70c

1.42 ± 0.16d

77.82

172.79

r14EGT

35.39 ± 8.23a

1.93 ± 0.77d

1.15 ± 0.36d

82.04

163.8

Trial 4

Mixed infection

Unchallenged control#

41.05 ± 16.08a

0 ± 0a

0 ± 0a

100

200

Challenged control

12.00 ± 9.50b

3.33 ± 0.47b

14.60 ± 1.96b

0

56.14

pVAX1 control

15.21 ± 8.64b

3.67 ± 0.47b

13.96 ± 3.02b

4.36

46.95

pET-32a tag protein control

15.49 ± 10.47b

3.6 ± 0.49b

13.44 ± 5.19b

7.89

48.06

pVAX1-14EGT

32.83 ± 8.52a

2.73 ± 0.44c

7.00 ± 0.90c

52.06

142.18

r14EGT

33.05 ± 7.88a

2.53 ± 0.50c

6.51 ± 0.86c

55.4

146.41

  1. 1. Value = mean ± S.D., n = 15. 2. Data comparisons were limited to within each individual trial and were not conducted across the four trials. 3. In the same column within a trial, data superscripts sharing the same letter (a–d) denote a nonsignificant difference (p > 0.05), while different letters a–d indicate a significant difference (p < 0.05). 4.
  2. *Trial 1 and trial 2 shared the data from the same unchallenged control.
  3. #Trial 3 and trial 4 shared the data from the same unchallenged control. 5. Mixed infection: challenge with the mixed oocyst of the above 3 Eimeria species (1 × 105 E. maxima, 1 × 105 E. acervulina and 5 × 104 E. tenella).