Figure 2
From: The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens
Phenotype of infected DF-1 cells. All experiments were conducted using DF-1 cells infected at MOI 0.1 and harvested 24 hpi. The cells were stained and analysed by flow cytometry. Data is shown as mean (SD) control (white), wt (blue), ΔF2 (red). Statistical analysis was calculated as One-Way ANOVA. Depicted are representative results from one of at least three individual experiments. A Live-dead-staining was performed with Zombie Aqua™ viability dye. Zombie-negative fraction of a population of single cells. (p < 0.0001) B Live cells were further characterized using a monoclonal antibody against nucleoprotein (NP) to stain infected cells. NP positive cells (p < 0.0001) as well as the expression of NP in the cells (as MFI NP, p < 0.0001). C Apoptotic cells were identified based on positivity for active Caspase 3 (p < 0.0001). Caspase expression was analysed in Zombie-negative, NP-positive cells. A population of naïve, Zombie-negative cells was used as control.