Figure 3

Expression of PB1-F2 altered survival period and clinical onset in chickens. A, B Kaplan Meier plotting of survival rates of inoculated (n = 7, A) and contact animals (n = 5, B), wt (blue), ΔF2 (red). Statistical analysis was done by Log-rank (Mantel-Cox) test (infected: p = 0.0287; contact: p = 0.0411) C, D Clinical scoring for inoculates (1–10) and contact birds (11–15) after infection with wt (C) or ΔF2 (D). Scores (0–3) were assigned as follows: 0: no symptoms, 1: one symptom, 2: multiple signs of disease, 3: death. 4 dpi 3 animals per group were sacrificed for necropsy and sampling (P). E Clinical scoring of chickens depicted as Mean with Standard deviation. Statistical analysis was performed as a two-way ANOVA with Šídák’s multiple comparisons test (p = 0.2850).